Module 2 Quiz: Terminology & DNA Markers | Bioinformatics Teaching Kit

Question 1

What is a Trace QC and why it is important in Sanger sequencing?

Sample Answer: Trace QC reflects the quality values of each base call in a Sanger sequencing chromatogram. It is important because it helps determine the reliability of the sequence data. Quality values of 20+ (Phred score ≥ 20) indicate good quality base calls with ≤ 1% error probability. Low Trace QC values suggest poor sequencing runs that may need to be repeated. Evaluating Trace QC ensures that only high-confidence sequence data is used for downstream analysis such as BLAST searches and species identification.

Question 2

Which marker best suited for bacterial Identification?

a. ITS b. 16S rRNA c. 18S rRNA

Question 3

ITS is situated between which gene?

a. 16S and 18S b. 18S and 28S c. 18S and 23S

Question 4

Average length of ideal primer should be?

a. 5 to 10bp b. 30 to 35bp c. 1 to 20bp d. 18 to 25bp

Question 5

ITS region is consisting of -

a. ITS1 b. ITS2 c. 5.8s d. All the above

Terminology & DNA Markers — Quiz