Data Analysis — Quiz Part 1

Sample Answer: FASTA is a text-based format for representing nucleotide or protein sequences. It consists of two parts: (1) a header line starting with '>' followed by a unique identifier, organism name, and gene/marker details; (2) one or more lines of the actual sequence data using standard nucleotide codes (A, T, G, C). Multiple sequences can be included in a single file, each starting with a new '>' header.

Sample Answer: No, two primers alone cannot reliably sequence more than 1600 bp. Sanger sequencing can produce clean sequences with a maximum read length of approximately 800–900 bp per primer. With two primers (one forward and one reverse), the maximum combined coverage would be around 1600–1800 bp, but only if there is sufficient overlap. For longer sequences, additional internal primers are required.